The purpose of this study was to assess thepsychometric properties of the Spanish Burnout Inventory(SBI) in a sample of Italian health professionals. The sampleconsisted of 695 participants: 453 nursing professionalsand 242 physicians. Regarding the gender, 451 werewomen (64.9%) and 244 were men (35.1%). The SBI iscomposed of 20 items distributed in four dimensions:Enthusiasm towards the job (5 items), Psychologicalexhaustion (4 items), Indolence (6 items) and Guilt (5 items).The factor structure was examined through confirmatoryfactor analysis. All items achieved appropriate values forboth statistical and psychometric parameters, just as thefour scales did. The four-factor model consistent with theSpanish original obtained a good data fit for the wholesample, showing factor invariance in both samples (i.e.,men vs. women). In conclusion, the SBI has adequatepsychometric properties to study the burnout syndromein Italian health professionals, regardless of their gender.