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Properties of a nuclear protein marker of human myeloid cell differentiation.

Authors
  • Goldberger, A
  • Hnilica, L S
  • Casey, S B
  • Briggs, R C
Type
Published Article
Journal
The Journal of biological chemistry
Publication Date
Apr 05, 1986
Volume
261
Issue
10
Pages
4726–4731
Identifiers
PMID: 3457010
Source
Medline
License
Unknown

Abstract

The Mr 55,000 nuclear antigen present in the human promyelocytic cell line HL-60 is a basic protein that is extracted from nuclei or chromatin by 0.35 M NaCl. The antigen is confined to the nucleus of the interphase HL-60 cell as judged by immunocytochemical localization but disperses throughout the cell during mitosis. The antigen was not detected in leukemic cell lines with blast cell properties or in cell lines representing other lineages. Additional cell lines (ML-1, ML-2, and U937) with myeloid cell characteristics similar to those of the HL-60 cells, which also differentiate in vitro, express the antigen. The presence of antigen in normal human myeloid cells in peripheral blood and bone marrow is consistent with its proposed role in nuclear events associated with normal human myeloid cell differentiation.

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