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PROPERTIES OF NEWLY INTRODUCED TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.

Authors
  • VENEMA, G
  • PRITCHARD, R H
  • VENEMA-SCHROEDER, T
Type
Published Article
Journal
Journal of bacteriology
Publication Date
Aug 01, 1965
Volume
90
Pages
343–346
Identifiers
PMID: 14329445
Source
Medline
Keywords
License
Unknown

Abstract

Venema, G. (Hammersmith Hospital, London, England), R. H. Pritchard, and T. Venema-Schröder. Properties of newly introduced transforming deoxyribonucleic acid in Bacillus subtilis. J. Bacteriol. 90:343-346. 1965.-In these experiments, deoxyribonucleic acid (DNA) with donor markers, re-extracted shortly after uptake by competent Bacillus subtilis cells, had abnormal physical properties as compared with DNA with resident markers. It was less sensitive to thermal denaturation, and its capacity to renaturation was reduced. DNA showing joint activity of donor and recipient markers (recombinant-type activity) had similar abnormal properties, revealing that, despite the appearance of recombinant-type activity, integration was not complete.

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