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Properties of liver mitochondria from iron-loaded rats

Authors
  • Hanstein, Walter G.
  • Sacks, Peter V.
  • Muller-Eberhard, Ursula
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Publication Date
Jan 01, 1975
Volume
67
Issue
3
Pages
1175–1184
Identifiers
DOI: 10.1016/0006-291X(75)90797-4
Source
Elsevier
Keywords
License
Unknown

Abstract

The increased iron content in livers from iron-loaded rats is almost exclusively confined to the mitochondria. The ten- to twenty-fold higher level of nonheme iron in such mitochondria decreases the respiratory control with pyruvate-malate, but not with 3-hydroxybutyrate or succinate as substrates, and has no effect on the capacity for phosphorylation and substrate oxidation. Iron-loaded mitochondria have a malondialdehyde level which is about three times higher than that of control mitochondria, even after repeated washings with bovine serum albumin and EDTA. This is suggestive of an on-going process of lipid oxidation presumably catalyzed by the accumulated iron. Differences between the present in vivo data and in vitro results obtained by others are discussed.

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