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Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.

Authors
  • Aström, J
  • Aström, A
  • Virtanen, A
Type
Published Article
Journal
The Journal of biological chemistry
Publication Date
Sep 05, 1992
Volume
267
Issue
25
Pages
18154–18159
Identifiers
PMID: 1355481
Source
Medline
License
Unknown

Abstract

A HeLa cell 3'-exonuclease with properties of a mammalian mRNA poly(A) tail-removing enzyme has been characterized. The exonuclease shows high specificity for the poly(A) tail, and it is single strand-specific and requires a 3'-hydroxyl group for its activity. During degradation 5'-AMP is liberated as a product, and a 3'-OH group is left on the last adenosine residue of the remaining poly(A) tail. The activity is inhibited by 5'-AMP and can be competed by poly(A)-containing mRNA or poly(A). Based on these findings we propose a reaction pathway for poly(A) tail removal catalyzed by the HeLa cell poly(A) tail-specific 3' exonuclease.

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