Affordable Access

Access to the full text

The promoter of tomato HISTIDINE DECARBOXYLASE A is fruit-specific, and its expression is stably maintained in fruits during ripening

Authors
  • Kim, Ah Young1
  • Kim, Hyun Min1
  • Ma, Sang Hoon1
  • Park, Seo Young1
  • Dat, Mai Thanh1
  • Jang, Geupil1
  • Joung, Young Hee1
  • 1 Chonnam National University, School of Biological Sciences and Technology, Gwangju, 61186, Republic of Korea , Gwangju (South Korea)
Type
Published Article
Journal
Plant Biotechnology Reports
Publisher
Springer Singapore
Publication Date
Jan 03, 2019
Volume
13
Issue
1
Pages
43–50
Identifiers
DOI: 10.1007/s11816-018-00512-1
Source
Springer Nature
Keywords
License
Yellow

Abstract

Identifying novel promoters with specific temporal and spatial expression patterns is crucial for crop biotechnology. In this study, we isolated a fruit-specific promoter in tomato, HISTIDINE DECARBOXYLASE A (SlHDC-A) promoter. Through RNA-seq and RT-PCR analysis, we found that SlHDC-A was predominantly expressed in fruits and that its expression was stable in fruits during ripening. These results suggest that the promoter of SlHDC-A might have the ability to determine fruit-specific gene expression. To test this possibility, we generated transgenic tomato transformed with SlHDC-A::GUS and 35S::GUS. Unlike 35S::GUS transgenic tomato with constitutive expression in various tissues, SlHDC-A::GUS transgenic plants showed fruit-specific expression of GUS. The intensity of GUS activity in fruits of SlHDC-A::GUS transgenic plants was approximately tenfold higher than that in fruits of 35S::GUS transgenic plants. The core region responsible for its fruit-specific expression was identified by promoter deletion analyses. Removal of the − 880 to − 577 region abolished the fruit-specific expression of SlHDC-A promoter. This suggests that the − 880 to − 577 region is the core region responsible for the fruit-specific expression of SlHDC-A. This finding was further supported by analysis of chimeric fusion promoter. Unlike 35S minimal promoter which had no activity to express GUS, the chimeric fusion promoter of the core region and 35S minimal promoter showed fruit-specific expression similar to intact SlHDC-A promoter. Collectively, these findings indicate that the promoter of SlHDC-A is fruit-specific and the − 880 to − 577 region is the core region of SlHDC-A promoter.

Report this publication

Statistics

Seen <100 times