Affordable Access

deepdyve-link
Publisher Website

Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds.

Authors
  • Ricotti, Leonardo
  • Polini, Alessandro
  • Genchi, Giada G
  • Ciofani, Gianni
  • Iandolo, Donata
  • Vazão, Helena
  • Mattoli, Virgilio
  • Ferreira, Lino
  • Menciassi, Arianna
  • Pisignano, Dario
Type
Published Article
Journal
Biomedical materials (Bristol, England)
Publication Date
Jun 01, 2012
Volume
7
Issue
3
Pages
35010–35010
Identifiers
DOI: 10.1088/1748-6041/7/3/035010
PMID: 22477772
Source
Medline
License
Unknown

Abstract

This study aims at investigating the behavior in terms of the proliferation and skeletal muscle differentiation capability of two myoblastic cell lines, C2C12 and H9c2, on both isotropic and anisotropic electrospun nanofibrous poly(hydroxybutyrate) (PHB) scaffolds, as well as on PHB films and polystyrene controls. After a careful characterization of the matrices in terms of surface morphology, surface roughness and mechanical properties, the proliferation rate and the capability of the two cell lines to form skeletal myotubes were evaluated. Genetic analyses were also performed in order to assess the differentiation level of the cells on the different substrates. We demonstrated that the aligned nanofibrous mesh decreases the proliferation activity and provides a higher differentiative stimulus. We also clarified how the nanofibrous substrate influences myotube formation, and quantified a series of myotube-related parameters for both C2C12 and H9c2 cells.

Report this publication

Statistics

Seen <100 times