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The prokaryotic community of subglacial bottom sediments of Antarctic Lake Untersee: Detection by cultural and direct microscopic techniques

Authors
  • A L, Mulyukin
  • E V, Demkina
  • N A, Manucharova
  • V N, Akimov
  • Dale Andersen
  • C, Mckay
  • V F, Gal Chenko
Type
Published Article
Journal
Microbiology
Publisher
Microbiology Society
Publication Date
Jan 01, 2014
Volume
83
Issue
1-2
Pages
77–84
Source
SETI Institute
License
Unknown

Abstract

The heterotrophic mesophilic microbial component was studied in microbial communities of the samples of frozen regolith collected from the glacier near Lake Untersee collected in 2011 during the joint Russian-American expedition to central Dronning Maud Land (Eastern Antarctica). Cultural techniques revealed high bacterial numbers in the samples. For enumeration of viable cells, the most probable numbers (MPN) method proved more efficient than plating on agar media. Fluorescent in situ hybridization with the ...

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