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Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configuration.

Authors
  • Meanwell, Richard J L
  • Shama, Gilbert
Type
Published Article
Journal
Bioresource Technology
Publisher
Elsevier
Publication Date
Sep 01, 2008
Volume
99
Issue
13
Pages
5634–5639
Identifiers
PMID: 18054224
Source
Medline
License
Unknown

Abstract

Streptomyces griseus was cultured in three different bioreactors in a medium containing chitin flakes. When a conventional bioreactor stirred by two sets of Rushton impellers and operated at high speed was used, the yield of streptomycin (3.1mg/l) was the highest observed and occurred at approximately 500 h. Cultivation of S. griseus in a bioreactor stirred at low speed by a U-shaped paddle resulted in a lower yield of streptomycin (1.8 mg/l) but this was achieved in a shorter period of time (400 h). Increasing the concentration of chitin from 5% to 10% w/v had no significant effect on either of these two parameters. The use of a novel vertical basket bioreactor in which the chitin flakes were contained within a wire mesh basket and were gently fluidised by air, enabled comparatively high yields of streptomycin (2.8 mg/l) in the relatively short time of 300 h.

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