Affordable Access

deepdyve-link
Publisher Website

Production of ROS by photosensitized anthracene under sunlight and UV-R at ambient environmental intensities.

Authors
  • Mujtaba, Syed Faiz
  • Dwivedi, Ashish
  • Mudiam, Mohana Krishna Reddy
  • Ali, Daoud
  • Yadav, Neera
  • Ray, Ratan Singh
Type
Published Article
Journal
Photochemistry and Photobiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
2011
Volume
87
Issue
5
Pages
1067–1076
Identifiers
DOI: 10.1111/j.1751-1097.2011.00955.x
PMID: 21668866
Source
Medline
License
Unknown

Abstract

The aim of this study was to analyze the photostability and phototoxicity mechanism of anthracene (ANT) in a human skin epidermal cell line (HaCaT) at ambient environmental intensities of sunlight/UV-R (UV-A and UV-B). Photomodification of ANT under sunlight/UV-R exposure produced two photoproducts, anthrone and 9,10 anthracenedione. Generation of (1)O(2), O(2)(•-) and (•)OH was measured under UV-R/sunlight exposure. Involvement of reactive oxygen species (ROS) was further substantiated by their quenching with free radical quenchers. Photodegradation of 2-deoxyguanosine and linoleic acid peroxidation showed that ROS were mainly responsible for ANT phototoxicity. ANT generates significant amount of intracellular ROS in cell line. Maximum cell viability (85%) was reduced under sunlight exposure (30 min). Results of MTT assay accord NRU assay. ANT (0.01 μg mL(-1)) induced cell-cycle arrest at G1 phase. RT-PCR demonstrated constitutive inducible mRNA expression of CYP 1A1 and 1B1 genes. Photosensitive ANT upregulates CYP 1A1 (2.2-folds) and 1B1 (4.1-folds) genes. Thus, the study suggests that ROS and DNA damage were mainly responsible for ANT phototoxicity. ANT exposure may be deleterious to human health at ambient environmental intensities reaching the earth's surface through sunlight.

Report this publication

Statistics

Seen <100 times