Affordable Access

Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae.

Authors
Type
Published Article
Journal
Journal of medical microbiology
Publication Date
Volume
27
Issue
3
Pages
215–224
Identifiers
PMID: 3193444
Source
Medline
License
Unknown

Abstract

The production of haemolysin from Treponema hyodysenteriae was increased by an improved culture method and by repeated incubation of spirochaetes suspended in a buffer containing RNA-core. Ion exchange chromatography on DEAE cellulose followed by gel filtration on Sephadex G100 yielded purified haemolysin free from extraneous protein, as judged by silver-stained polyacrylamide gels. The mol. wt of the purified haemolysin, determined by gel filtration was 19,000, a value similar to that of streptolysin S, but much lower than that previously reported.

Statistics

Seen <100 times