Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae.
- Published Article
Journal of medical microbiology
- Publication Date
Nov 01, 1988
The production of haemolysin from Treponema hyodysenteriae was increased by an improved culture method and by repeated incubation of spirochaetes suspended in a buffer containing RNA-core. Ion exchange chromatography on DEAE cellulose followed by gel filtration on Sephadex G100 yielded purified haemolysin free from extraneous protein, as judged by silver-stained polyacrylamide gels. The mol. wt of the purified haemolysin, determined by gel filtration was 19,000, a value similar to that of streptolysin S, but much lower than that previously reported.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/3193444