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Production of perdeuterated fucose from glyco-engineered bacteria

Authors
  • Gajdos, Lukas1, 2, 3
  • Forsyth, V Trevor1, 2, 4
  • Blakeley, Matthew P5
  • Haertlein, Michael1, 2
  • Imberty, Anne3
  • Samain, Eric3
  • Devos, Juliette M1, 2
  • 1 Life Sciences Group, France , (France)
  • 2 Partnership for Structural Biology (PSB), France , (France)
  • 3 Université Grenoble Alpes, France , (France)
  • 4 Faculty of Natural Sciences
  • 5 Large Scale Structures Group, France , (France)
Type
Published Article
Journal
Glycobiology
Publisher
Oxford University Press
Publication Date
Jun 27, 2020
Volume
31
Issue
2
Pages
151–158
Identifiers
DOI: 10.1093/glycob/cwaa059
PMID: 32601663
PMCID: PMC7874385
Source
PubMed Central
Keywords
License
Unknown

Abstract

l -Fucose and l -fucose-containing polysaccharides, glycoproteins or glycolipids play an important role in a variety of biological processes. l -Fucose-containing glycoconjugates have been implicated in many diseases including cancer and rheumatoid arthritis. Interest in fucose and its derivatives is growing in cancer research, glyco-immunology, and the study of host–pathogen interactions. l -Fucose can be extracted from bacterial and algal polysaccharides or produced (bio)synthetically. While deuterated glucose and galactose are available, and are of high interest for metabolic studies and biophysical studies, deuterated fucose is not easily available. Here, we describe the production of perdeuterated l -fucose, using glyco-engineered Escherichia coli in a bioreactor with the use of a deuterium oxide-based growth medium and a deuterated carbon source. The final yield was 0.2 g L−1 of deuterated sugar, which was fully characterized by mass spectrometry and nuclear magnetic resonance spectroscopy. We anticipate that the perdeuterated fucose produced in this way will have numerous applications in structural biology where techniques such as NMR, solution neutron scattering and neutron crystallography are widely used. In the case of neutron macromolecular crystallography, the availability of perdeuterated fucose can be exploited in identifying the details of its interaction with protein receptors and notably the hydrogen bonding network around the carbohydrate binding site.

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