Production of cloned human leukocyte interferon by Bacillus subtilis: optimal production is connected with restrained growth.

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Production of cloned human leukocyte interferon by Bacillus subtilis: optimal production is connected with restrained growth.

Publication Date
Aug 01, 1985
Source
PMC
Keywords
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Unknown

Abstract

Bacillus subtilis, transformed with a plasmid containing the human alpha-2 (leukocyte) interferon gene, was cultivated in batch and continuous culture in a complex medium. In continuous culture with dissolved oxygen of less than 10% of air saturation, the extracellular interferon titer decreased sharply when the growth rate was lower or higher than the optimal one (mu = 0.14 h-1). Thus, a relatively low growth rate was best for extracellular interferon production, and oxygen limitation enhanced interferon production. The mean output rate in batch culture after successful harvest was 20 X 10(6) IU/liter per h and the maximal output rate in continuous culture was 14 X 10(6) IU/liter per h.

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