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Production of Murine Macrophages from Hoxb8-Immortalized Myeloblasts: Utility and Use in the Context of Salmonella Infection.

Authors
  • Fang, Ziyan1
  • Lagier, Margaux1
  • Méresse, Stéphane2
  • 1 Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France. , (France)
  • 2 Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France. [email protected] , (France)
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2021
Volume
2182
Pages
117–126
Identifiers
DOI: 10.1007/978-1-0716-0791-6_11
PMID: 32894491
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Salmonella enterica is a Gram-negative intracellular pathogen that causes a range of life-threatening diseases in humans and animals worldwide. In a systemic infection, the ability of Salmonella to survive/replicate in macrophages, particularly in the liver and spleen, is crucial for virulence. Transformed macrophage cell lines and primary macrophages prepared from mouse bone marrow are commonly used models for the study of Salmonella infection. However, these models raise technical or ethical issues that highlight the need for alternative methods. This chapter describes a technique for immortalizing early hematopoietic progenitor cells derived from wild-type or transgenic mice and using them to produce macrophages. It validates, through a specific example, the interest of this cellular approach for the study of Salmonella infection.

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