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Production of functional rat liver PSP protein in Escherichia coli.

Authors
Type
Published Article
Journal
Cellular and molecular life sciences : CMLS
Publication Date
Volume
55
Issue
1
Pages
131–134
Identifiers
PMID: 10065159
Source
Medline
License
Unknown

Abstract

An efficient Escherichia coli expression system for the production of a perchloric acid-soluble protein (PSP) has been constructed. Complementary DNA encoding PSP was inserted into an inducible bacterial expression vector pGEX-4T-1. After the plasmid introduced into E. coli was expressed by isopropyl 1-thio-beta-D-galaetopyranoside (IPTG), the recombinant product was purified by glutathione-Sepharose 4B affinity chromatography. The purified product showed the expected NH2-terminal sequence, but the translation inhibitory activity of this product was 10 times lower compared with that of authentic PSP isolated from rat liver.

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