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Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1

Authors
  • Maia, Maria de Mascena Diniz
  • Morais, Marcia Maria Camargo de
  • Morais Jr., Marcos Antonio de
  • Melo, Eduardo Henrique Magalhães
  • Lima Filho, José Luiz de
Publication Date
Dec 01, 1999
Source
Scientific Electronic Library Online - Brazil
Keywords
License
Unknown
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Abstract

A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.

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