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Production of cybrids in Brassicaceae species.

Authors
  • Vasylenko, Maksym
  • Ovcharenko, Olga
  • Gleba, Yuri
  • Kuchuk, Nikolay
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2006
Volume
318
Pages
219–232
Identifiers
PMID: 16673919
Source
Medline
License
Unknown

Abstract

This chapter describes a method of cytoplasm transfer within the brassicaceae family through Ca-PEG-mediated protoplast fusion. The method includes a protocol of nonmutagenic albinism induction based on spectinomycin-induced plastid ribosome deficiency (PRD). The proposed application of spectinomycin-mediated albinism allows speeding up creation of albino lines, as well as of hybrid production with substituted cytoplasm. According to described method cybrids between Orychophragmus violaceus and Brassica napus, O. violaceus and Lesquerella fendleri have been produced. Methods of further molecular analysis are also presented. The time-scale and reliability of described methods are indicated.

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