Affordable Access

Access to the full text

Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

Authors
  • Singina, G. N.1
  • Sergiev, P. V.2, 3, 4
  • Lopukhov, A. V.1
  • Rubtsova, M. P.4
  • Taradajnic, N. P.1
  • Ravin, N. V.5
  • Shedova, E. N.1
  • Taradajnic, T. E.1
  • Polejaeva, I. A.6
  • Dozev, A. V.1
  • Brem, G.7
  • Dontsova, O. A.3, 4, 8, 9
  • Zinovieva, N. A.1
  • 1 Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia , Podolsk (Russia)
  • 2 Institute of Functional Genomics, Moscow State University, Moscow, Russia , Moscow (Russia)
  • 3 Center of Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo, Russia , Skolkovo (Russia)
  • 4 Faculty of Chemistry, Moscow State University, Moscow, Russia , Moscow (Russia)
  • 5 Research Center of Biotechnology, Moscow, Russia , Moscow (Russia)
  • 6 Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA , Logan (United States)
  • 7 Department of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria , Vienna (Austria)
  • 8 Belozersky Research Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia , Moscow (Russia)
  • 9 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia , Moscow (Russia)
Type
Published Article
Journal
Doklady Biochemistry and Biophysics
Publisher
Pleiades Publishing
Publication Date
Mar 10, 2021
Volume
496
Issue
1
Pages
48–51
Identifiers
DOI: 10.1134/S1607672921010099
Source
Springer Nature
Keywords
License
Green

Abstract

AbstractSomatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.

Report this publication

Statistics

Seen <100 times