Affordable Access

Production and characterization of an extracellular lipase from Candida guilliermondii.

Authors
  • Oliveira, Anne Caroline Defranceschi
  • Fernandes, Maria Luiza
  • Mariano, André Bellin
Type
Published Article
Journal
Brazilian Journal of Microbiology
Publisher
Elsevier
Publication Date
Jan 01, 2014
Volume
45
Issue
4
Pages
1503–1511
Identifiers
PMID: 25763060
Source
Medline
Keywords
License
Unknown

Abstract

Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL(-1)) in the presence of 5 mmol L(-1) NaCl at 30 °C and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL(-1). The produced enzyme was applied in the oleic acid esterification under different solvents: hexane, cyclohexane or cyclohexanone) and different acid:alcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 1:9 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.

Report this publication

Statistics

Seen <100 times