Affordable Access

Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase.

Authors
  • Fanta, M
  • Zhang, H
  • Bernstein, N
  • Glover, M
  • Karimi-Busheri, F
  • Weinfeld, M
Type
Published Article
Journal
Hybridoma
Publication Date
Aug 01, 2001
Volume
20
Issue
4
Pages
237–242
Identifiers
PMID: 11604109
Source
Medline
License
Unknown

Abstract

Polydeoxyribonucleotide kinase (PNK) is a mammalian DNA repair enzyme that has the capacity to phosphorylate 5' DNA termini and dephosphorylate 3' DNA termini. A series of murine monoclonal antibodies (MAbs) was raised against the full-length recombinant human PNK. Seven of these antibodies were selected and characterized by enzyme immunoassay, Western blot analysis, and their capacity to immunoprecipitate PNK. The epitope location was defined by cyanogen bromide digestion and by using a truncated PNK for Western blot analysis. All of the MAbs recognize a single 60-kDa protein in human cell extracts. PNKs from calf, monkey, and Chinese hamster cell and tissue extracts were also detected by some or all of the MAbs. These antibodies can be successfully used for the cellular, biochemical, and functional analysis of PNK in different mammalian cell lines.

Report this publication

Statistics

Seen <100 times