Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Probability of antibody formation against circumsporozoite protein of Plasmodium vivax among Korean malaria patients.

Authors
  • Nam, Ho-Woo1
  • Song, Kyoung Ju1
  • Ahn, Hye Jin1
  • Yang, Zhaoshou1
  • Chong, Chom-Kyu2
  • Cho, Pyo Yun3
  • Ahn, Seong Kyu3
  • Kim, Tong-Soo3
  • 1 Department of Parasitology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. , (North Korea)
  • 2 GenBody Inc., Cheonan 330-714, Korea. , (North Korea)
  • 3 Department of Tropical Medicine and Parasitology, Inha University School of Medicine, Incheon 400-712, Korea. , (North Korea)
Type
Published Article
Journal
The Korean journal of parasitology
Publication Date
Apr 01, 2014
Volume
52
Issue
2
Pages
143–149
Identifiers
DOI: 10.3347/kjp.2014.52.2.143
PMID: 24850957
Source
Medline
Keywords
License
Unknown

Abstract

To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic DNA of patient's blood, 2 kinds of CSPs were identified to belong to a VK210 type, which is the dominant repeating of GDRA(D/A)GQPA, and named as PvCSPA and PvCSPB. Recombinantly expressed his-tagged PvCSPA or PvCSPB in Escherichia coli reacted well against sera of patients in western blot, with the detecting rate of 47.9% (58/121), which included 15 cases positive for PvCSPA, 6 cases positive for PvCSPB, and 37 cases for both. The mixture of PvCSPA and PvCSPB was loaded to a rapid diagnostic test kit (RDT) and applied with the same set of patient sera, which resulted in detection rates of 57.0% (69/121). When the protein sequences of PvCSPA were compared with those of P. vivax in endemic regions of India and Uganda, they were compatibly homologous to PvCSPA with minor mutations. These results suggested that the recombinant PvCSPA and PvCSPB loaded RDT may be a milestone in latent diagnosis which has been a hot issue of domestic malaria and important for radical therapy in overlapped infections with P. falciparum in tropical and subtropical areas. During the biological process of malarial infection, exposure of CSP to antigen-antibody reaction up to 57.0% is the first report in Korea.

Report this publication

Statistics

Seen <100 times