Affordable Access

Pro-major basic protein has three types of sugar chains at the pro-portion.

Authors
  • Shikata, Y
  • Hayashi, Y
  • Yoshimatsu, K
  • Ohya, Y
  • Seto, T
  • Fukushima, K
  • Yoshida, Y
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Jun 04, 1993
Volume
1163
Issue
3
Pages
243–249
Identifiers
PMID: 8507662
Source
Medline
License
Unknown

Abstract

The amino-acid sequence of purified recombinant pro-major basic protein from Chinese hamster kidney cells was determined to verify the primary structure and glycosylation sites. Reduced and S-carboxamidemethylated protein was first digested with Achromobacter proteinase I. Each peptide was characterized by amino-acid analysis and amino-acid sequence analysis. We could identify all the peptides which were expected from the pro-major basic protein cDNA sequence. Sequence analysis and deglycosylation study revealed that Ser-8, Thr-9, Ser-46 and Asn-70 were glycosylated. The results indicated that proMBP has three types of sugar chains, O-glycoside, N-glycoside and glycosaminoglycan, in the pro-portion.

Report this publication

Statistics

Seen <100 times