Prior use of the neutral red assay and reduction of total protein determination in 96-well plate assays.
- Published Article
Toxicology in Vitro
- Publication Date
Jan 01, 1990
The sequential use of the neutral red and protein assays in 96-well plates leads to approximately a 50% underestimation of the amount of protein. In absolute terms, more protein is lost from wells having more protein, but within a cell type the reduction appears to be proportional to the amount of protein. This should be taken into account when interpreting data obtained in this way.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/20837418