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Prior use of the neutral red assay and reduction of total protein determination in 96-well plate assays.

Authors
Type
Published Article
Journal
Toxicology in Vitro
0887-2333
Publisher
Elsevier
Publication Date
Volume
4
Issue
3
Pages
211–212
Identifiers
PMID: 20837418
Source
Medline
License
Unknown

Abstract

The sequential use of the neutral red and protein assays in 96-well plates leads to approximately a 50% underestimation of the amount of protein. In absolute terms, more protein is lost from wells having more protein, but within a cell type the reduction appears to be proportional to the amount of protein. This should be taken into account when interpreting data obtained in this way.

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