Affordable Access

deepdyve-link
Publisher Website

Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation.

Authors
  • Knudsen, Jonas R1
  • Steenberg, Dorte E2
  • Hingst, Janne R2
  • Hodgson, Lorna R3
  • Henriquez-Olguin, Carlos2
  • Li, Zhencheng2
  • Kiens, Bente2
  • Richter, Erik A2
  • Wojtaszewski, Jørgen F P2
  • Verkade, Paul3
  • Jensen, Thomas E4
  • 1 Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark; Laboratory of Microsystems 2, Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne, Batiment BM, 1015, Lausanne, Switzerland. , (Switzerland)
  • 2 Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark. , (Denmark)
  • 3 School of Biochemistry, University of Bristol, Biomedical Sciences Building, University Walk, BS8 1TD, Bristol, United Kingdom. , (United Kingdom)
  • 4 Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark. Electronic address: [email protected] , (Denmark)
Type
Published Article
Journal
Molecular Metabolism
Publisher
Elsevier BV
Publication Date
Sep 01, 2020
Volume
39
Pages
100998–100998
Identifiers
DOI: 10.1016/j.molmet.2020.100998
PMID: 32305516
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown. Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 h followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4 h post-exercise before and after a 2-hour hyperinsulinemic-euglycemic clamp. A detailed microscopy-based analysis of GLUT4 distribution within seven different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments. An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle. Copyright © 2020 The Author(s). Published by Elsevier GmbH.. All rights reserved.

Report this publication

Statistics

Seen <100 times