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Primary structure of a mouse mastocytoma proteoglycan core protein.

Authors
  • L, Kjellén
  • I, Pettersson
  • P, Lillhager
  • Ml, Steen
  • U, Pettersson
  • P, Lehtonen
  • T, Karlsson
  • Erkki Ruoslahti
  • L, Hellman
Type
Published Article
Journal
Biochemical Journal
Publisher
Portland Press
Volume
263
Issue
1
Pages
105–113
Source
Ruoslahti Lab
License
Unknown

Abstract

The complete nucleotide sequence of a mouse mastocytoma proteoglycan core protein mRNA was determined. The mRNA, estimated to contain 1.1 kb, encodes a protein with an Mr of 16715. A 21-amino acid-residue region of the protein is composed of alternating serine and glycine residues. Southern-blot analysis of mouse genomic DNA with cDNA containing sequences corresponding to the Ser-Gly repeat region revealed more than 15 gene fragments. Hybridization with a probe corresponding to the N-terminal portion of the core protein identified two fragments, and cDNA covering the C-terminal part of the core protein and the 3 untranslated part of the mRNA hybridized to a single fragment. Antibodies against the core protein, obtained after immunization of rabbits with a fusion protein, reacted with both chondroitin sulphate proteoglycans and heparin proteoglycans produced by the tumour. In immunoblotting of a microsomal fraction from the mastocytoma, the antiserum recognized a single protein (Mr 17,000), which probably represents the core protein before glycosylation.

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