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Prevalence of two tumor necrosis factor gene polymorphisms in premature infants with early onset sepsis.

Authors
  • Schueller, A C1
  • Heep, A
  • Kattner, E
  • Kroll, M
  • Wisbauer, M
  • Sander, J
  • Bartmann, P
  • Stuber, F
  • 1 Department of Neonatology, University of Bonn, Bonn, Germany. , (Germany)
Type
Published Article
Journal
Biology of the neonate
Publication Date
Jan 01, 2006
Volume
90
Issue
4
Pages
229–232
Identifiers
PMID: 17108674
Source
Medline
Language
English
License
Unknown

Abstract

Tumor necrosis factor (TNF) is a central mediator of sepsis. The NcoI polymorphism within the TNF locus has been described as a prognostic marker for mortality in adult patients with sepsis. The aim of our study was to investigate the genotype and allele distribution of 2 TNF gene polymorphisms in preterm infants <32 weeks of gestational age, who developed early-onset sepsis. A double-blinded retrospective cohort study was carried out on stored Guthrie blood spot cards with group A including 67 premature infants <32 weeks of gestational age with proven early-onset sepsis and group B including 102 healthy newborn infants (>32 + 0 weeks of gestation). The genotype andallele distribution of the study population were also compared to reference groups of healthy adult volunteers (n = 252 for TNF-beta NcoI and n = 233 for -308 TNF-alpha promoter). Polymorphisms of the TNF-alpha promoter -308 region and the NcoI site of the TNF-beta gene were assessed using PCR followed by melting curve analysis or NcoI digestion. The groups were compared by estimation of Hardy-Weinberg equilibrium. The overall allele frequency and genotype distribution of the -308 TNF-alpha and NcoI polymorphism of the TNF-beta gene were comparable to the values found in the controls. The study results suggest that none of the analyzed TNF gene polymorphisms may serve as a prognostic marker for preterm infants at high risk of sepsis. Copyright (c) 2006 S. Karger AG, Basel.

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