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Pretreatment of garden biomass by alkali-assisted ultrasonication: effects on enzymatic hydrolysis and ultrastructural changes.

Authors
  • Gabhane, Jagdish
  • William, Spm Prince
  • Vaidya, Atul Narayan
  • Anand, Duraisamy
  • Wate, Satish
Type
Published Article
Journal
Journal of environmental health science & engineering
Publication Date
Jan 01, 2014
Volume
12
Pages
76–76
Identifiers
DOI: 10.1186/2052-336X-12-76
PMID: 24843790
Source
Medline
Keywords
License
Unknown

Abstract

The present investigation aims at studying the effectiveness of alkali-assisted ultrasonication on pretreatment of garden biomass (GB). Dry and powdered GB suspended in 1% NaOH was ultrasonicated for 15, 30 and 60 minutes at a frequency of 25 KHZ. The mode of action and effectiveness of alkali-assisted ultrasonication on GB was established through microscopic, scanning electron microscopic and X-ray diffraction studies. A perusal of results showed that alkali-assisted ultrasonication led to fibrillation of GB which ultimately facilitated enzymatic hydrolysis. The results also indicated that alkali-assisted ultrasonication is an efficient means of pretreatment of GB at moderate (45-50°C) working temperature and low (1%) concentration of alkali. The yield of reducing sugar after enzymatic hydrolysis increased almost six times as compared to control due to alkali-assisted ultrasonication.

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