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Pre-treatment with D942, a furancarboxylic acid derivative, increases desiccation tolerance in an anhydrobiotic tardigrade Hypsibius exemplaris.

Authors
  • Kondo, Koyuki1
  • Mori, Masaru1, 2
  • Tomita, Masaru1, 2
  • Arakawa, Kazuharu1, 2, 3
  • 1 Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan. , (Japan)
  • 2 Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa, Japan. , (Japan)
  • 3 Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan. , (Japan)
Type
Published Article
Journal
FEBS Open Bio
Publisher
Wiley (John Wiley & Sons)
Publication Date
Sep 01, 2020
Volume
10
Issue
9
Pages
1774–1781
Identifiers
DOI: 10.1002/2211-5463.12926
PMID: 32623826
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The tardigrade Hypsibius exemplaris can undergo anhydrobiosis. Several chemicals that inhibit successful anhydrobiosis in H. exemplaris have been identified, and these chemicals inhibit the activity of signaling molecules. In the present study, we investigated whether upregulation of the activity of these signaling molecules could improve desiccation tolerance of H. exemplaris. Pre-treatment with an indirect activator of AMP-activated protein kinase [AMPK; which directly inhibits mammalian NAD(P)H dehydrogenase [quinone] 1 [NQO1] of mitochondrial complex I (D942)] significantly improved desiccation tolerance of H. exemplaris, whereas a direct activator of AMPK did not. To elucidate the underlying molecular mechanisms, we examined the proteome of tardigrades treated with D942. Two proteins, putative glutathione S-transferase and pirin-like protein, were upregulated by treatment. Both of these proteins are known to be associated with the response to oxidative stress. One of the downregulated proteins was serine/threonine-proteinphosphatase 2A (PP2A) 65-kDa regulatory subunit A alpha isoform, and it is interesting to note that PP2A activity was previously suggested to be required for successful anhydrobiosis in H. exemplaris. Taken together, our results suggest that D942 treatment may partially induce responses common to those of desiccation stress. The identification of a chemical that improves desiccation tolerance of H. exemplaris may facilitate further investigation into desiccation tolerance mechanisms. © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

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