Affordable Access

deepdyve-link
Publisher Website

Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina.

Authors
  • Estrada, Cecilia S M Lucero1
  • Alcaráz, Lucia E2
  • Satorres, Sara E2
  • Manfredi, Eduardo3
  • Velázquez, Lidia Del C1
  • 1 Microbiología General, Universidad Nacional de San Luis, San Luis, Argentina. , (Argentina)
  • 2 Bacteriología y Virología, Área Microbiología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina. , (Argentina)
  • 3 Servicio Fisiopatogenia, Departamento de Bacteriología, INEI-ANLIS "Dr. Carlos G. Malbrán", Ciudad Autónoma de Buenos Aires, Argentina. , (Argentina)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Dec 01, 2013
Volume
44
Issue
4
Pages
1155–1161
Identifiers
DOI: 10.1590/S1517-83822014005000001
PMID: 24688505
Source
Medline
Keywords
License
Unknown

Abstract

An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers' intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.

Report this publication

Statistics

Seen <100 times