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Preparative separation of intact incomplete and empty adenovirus type 2 particles.

Authors
  • Tóth, M
  • Taródi, B
  • Béládi, I
Type
Published Article
Journal
Acta virologica
Publication Date
Jul 01, 1982
Volume
26
Issue
4
Pages
217–220
Identifiers
PMID: 6182779
Source
Medline
License
Unknown

Abstract

Complete adenovirus type 2 virions containing the whole genome were resistant to while empty capsids lacking DNA were breakable at a separation procedure involving customary CsCl gradient centrifugation. A purification method was developed using CsCl density gradient centrifugation in 5% glycerol which retained the integrity of incomplete particles as well as empty capsids. This procedure enabled to study the biological and physicochemical properties of different adenovirus type 2 particles.

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