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Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies.

Authors
  • Saito, T
  • Yasukawa, K
  • Suzuki, H
  • Futatsugi, K
  • Fukunaga, T
  • Yokomizo, C
  • Koishihara, Y
  • Fukui, H
  • Ohsugi, Y
  • Yawata, H
Type
Published Article
Journal
Journal of immunology (Baltimore, Md. : 1950)
Publication Date
Jul 01, 1991
Volume
147
Issue
1
Pages
168–173
Identifiers
PMID: 2051018
Source
Medline
License
Unknown

Abstract

Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One of the clones, RS13, produced IgG2a isotype that was capable of inhibiting IL-6 activity. ELISA for the quantitation of smIL-6R was established, which could detect smIL-6R in a quantity as low as 1 ng/ml.

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