Affordable Access

Publisher Website

Preparation of Proteoglycan Mimetic Graft Copolymers.

Authors
  • Kipper, Matt J1
  • Place, Laura W2
  • 1 Department of Chemical and Biological Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO, 80526-1370, USA. [email protected].
  • 2 Department of Chemical and Biological Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO, 80526-1370, USA.
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2016
Volume
1367
Pages
69–86
Identifiers
DOI: 10.1007/978-1-4939-3130-9_7
PMID: 26537466
Source
Medline
Keywords
License
Unknown

Abstract

Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains. The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans. By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities. The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end. Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.

Report this publication

Statistics

Seen <100 times