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Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders.

Authors
  • Rahmoune, Hassan1
  • Guest, Paul C2
  • 1 Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK. [email protected]
  • 2 Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil. , (Brazil)
Type
Published Article
Journal
Advances in experimental medicine and biology
Publication Date
Jan 01, 2017
Volume
974
Pages
299–303
Identifiers
DOI: 10.1007/978-3-319-52479-5_28
PMID: 28353249
Source
Medline
Keywords
License
Unknown

Abstract

Peripheral blood mononuclear cells (PBMCs) have been used as a surrogate model of brain function in studies of psychiatric disorders, such as schizophrenia. This chapter describes the preparation of PBMCs from whole blood using a density gradient/cell culture protocol. This includes collection of the PBMC culture media and preparation of cell extracts in order to provide the raw materials for proteomic analyses.

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