Affordable Access

deepdyve-link
Publisher Website

Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders.

Authors
  • Rahmoune, Hassan1
  • Guest, Paul C2
  • 1 Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK. [email protected]
  • 2 Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil. , (Brazil)
Type
Published Article
Journal
Advances in experimental medicine and biology
Publication Date
Jan 01, 2017
Volume
974
Pages
299–303
Identifiers
DOI: 10.1007/978-3-319-52479-5_28
PMID: 28353249
Source
Medline
Keywords
License
Unknown

Abstract

Peripheral blood mononuclear cells (PBMCs) have been used as a surrogate model of brain function in studies of psychiatric disorders, such as schizophrenia. This chapter describes the preparation of PBMCs from whole blood using a density gradient/cell culture protocol. This includes collection of the PBMC culture media and preparation of cell extracts in order to provide the raw materials for proteomic analyses.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. This record was last updated on 06/09/2018 and may not reflect the most current and accurate biomedical/scientific data available from NLM. The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/28353249
Report this publication

Statistics

Seen <100 times