Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders.
- Authors
- Type
- Published Article
- Journal
- Advances in experimental medicine and biology
- Publication Date
- Jan 01, 2017
- Volume
- 974
- Pages
- 299–303
- Identifiers
- DOI: 10.1007/978-3-319-52479-5_28
- PMID: 28353249
- Source
- Medline
- Keywords
- License
- Unknown
Abstract
Peripheral blood mononuclear cells (PBMCs) have been used as a surrogate model of brain function in studies of psychiatric disorders, such as schizophrenia. This chapter describes the preparation of PBMCs from whole blood using a density gradient/cell culture protocol. This includes collection of the PBMC culture media and preparation of cell extracts in order to provide the raw materials for proteomic analyses.