Affordable Access

deepdyve-link
Publisher Website

Preparation of monodispersed chitosan microspheres and in situ encapsulation of BSA in a co-axial microfluidic device.

Authors
  • Xu, J H
  • Li, S W
  • Tostado, C
  • Lan, W J
  • Luo, G S
Type
Published Article
Journal
Biomedical Microdevices
Publisher
Springer-Verlag
Publication Date
Feb 01, 2009
Volume
11
Issue
1
Pages
243–249
Identifiers
DOI: 10.1007/s10544-008-9230-3
PMID: 18810642
Source
Medline
License
Unknown

Abstract

This work describes a novel microfluidic method to prepare monodispersed chitosan microspheres by using the solvent extraction method. Our strategy is that a chitosan/acetic acid aqueous solution is emulsified in an organic phase containing the extractant by using the co-flowing shear method in a co-axial microfluidic device. The formed droplets are in situ solidified within a synthesizing channel by the extraction of acetic acid from the chitosan aqueous droplets to the organic solution. Based on this approach, the size of chitosan microspheres can be successfully controlled from 100 mum to 700 mum in diameter with a variation of less than 4%. Furthermore, high loading efficiency (>95%) of Bovine serum albumin (BSA) can be in situ encapsulated. The present method has the advantages of actively controlling the droplet diameter, narrow size distribution, good sphericity, and having a simple and low cost process, with a high throughput. This approach for the preparation of chitosan microspheres will provide many potential applications for pharmaceutical area.

Report this publication

Statistics

Seen <100 times