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Preparation and evaluation of 400μm I.D. polymer-based hydrophilic interaction chromatography monolithic columns with high column efficiency.

Authors
  • Liu, Chusheng1
  • Li, Haibin1
  • Wang, Qiqin1
  • Crommen, Jacques2
  • Zhou, Haibo3
  • Jiang, Zhengjin4
  • 1 Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China. , (China)
  • 2 Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; Department of Pharmaceutical Sciences, University of Liege, CHU B36, B-4000 Liege, Belgium. , (Belgium)
  • 3 Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: [email protected] , (China)
  • 4 Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Aug 04, 2017
Volume
1509
Pages
83–90
Identifiers
DOI: 10.1016/j.chroma.2017.06.034
PMID: 28629939
Source
Medline
Keywords
License
Unknown

Abstract

The quest for higher column efficiency is one of the major research areas in polymer-based monolithic column fabrication. In this research, two novel polymer-based HILIC monolithic columns with 400μm I.D.×800μm O.D. were prepared based on the thermally initiated co-polymerization of N,N-dimethyl-N-(3-methacrylamidopropyl)-N-(3-sulfopropyl) ammonium betaine (SPP) and ethylene glycol dimethacrylate (EDMA) or N,N'-methylenebisacrylamide (MBA). In order to obtain a satisfactory performance in terms of column permeability, mechanical stability, efficiency and selectivity, the polymerization parameters were systematically optimized. Column efficiencies as high as 142, 000 plates/m and 120, 000 plates/m were observed for the analysis of neutral compounds at 0.6mm/s on the poly(SPP-co-MBA) and poly(SPP-co-EDMA) monoliths, respectively. Furthermore, the Van Deemter plots for thiourea on the two monoliths were compared with that on a commercial silica based ZIC-HILIC column (3.5μm, 200Å, 150mm×300μm I.D.) using ACN/H2O (90/10, v/v) as the mobile phase at room temperature. It was noticeable that the Van Deemter curves for both monoliths, particularly the poly(SPP-co-MBA) monolith, are significantly flatter than that obtained for the ZIC-HILIC column, which indicates that in spite of their larger internal diameters, they yield better overall efficiency, with less peak dispersion, across a much wider range of usable linear velocities. A clearly better separation performance was also observed for nucleobases, nucleosides, nucleotides and small peptides on the poly(SPP-co-MBA) monolith compared to the ZIC-HILIC column. It is particularly worth mentioning that these 400μm I.D. polymer-based HILIC monolithic columns exhibit enhanced mechanical strength owing to the thicker capillary wall of the fused-silica capillaries.

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