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Preparation of cuticles from unhatched first-instar Drosophila larvae.

Authors
  • Stern, David L
  • Sucena, Elio
Type
Published Article
Journal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory
Publication Date
Sep 01, 2011
Volume
2011
Issue
9
Identifiers
DOI: 10.1101/pdb.prot065532
PMID: 21880820
Source
Medline
License
Unknown

Abstract

The finely sculpted cuticle of Drosophila carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. Studies of the Drosophila cuticle have focused mainly on first-instar larvae and adult cuticular morphology. This protocol describes the preparation of cuticles from larvae that have not yet hatched from the egg. It is designed for sampling all eggs laid by one or more females. This can be particularly useful, for example, when a mutation produces embryos that are unable to hatch from the egg.

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