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The preparation of a cold-water soluble polysaccharide from Grifola frondosa and its inhibitory effects on MKN-45 cells.

Authors
  • Liu, Chao1, 2
  • Ji, Hai-Yu3, 4
  • Wu, Peng1, 2
  • Yu, Juan1, 2
  • Liu, An-Jun5
  • 1 State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, 300457, Tianjin, People's Republic of China. , (China)
  • 2 QingYunTang Biotech (Beijing) Co., Ltd, Beijing Economic-Technological Development Area, No. 14, Zhonghe Street, 100176, Beijing, China. , (China)
  • 3 State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, 300457, Tianjin, People's Republic of China. [email protected] , (China)
  • 4 QingYunTang Biotech (Beijing) Co., Ltd, Beijing Economic-Technological Development Area, No. 14, Zhonghe Street, 100176, Beijing, China. [email protected] , (China)
  • 5 State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, 300457, Tianjin, People's Republic of China. [email protected] , (China)
Type
Published Article
Journal
Glycoconjugate Journal
Publisher
Springer-Verlag
Publication Date
Aug 01, 2020
Volume
37
Issue
4
Pages
413–422
Identifiers
DOI: 10.1007/s10719-020-09932-0
PMID: 32556780
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatography (HPGPC), fourier-transform infrared spectroscopy (FT-IR), and ion chromatography (IC) results showed that GFP-4 was a 1.09 × 106 Da neutral hetero polysaccharide with pyranose rings, and α- and β-type glycosidic linkages that contained galactose, glucose, and mannose at a molar ratio of 1.00:3.45:1.19. MTT results indicated that GFP-4 significantly inhibited the proliferation of MKN-45 cells in a concentration-dependent manner. The H&E staining and Hoechst 33342/PI double staining results showed that GFP-4-treated MKN-45 cells were subjected to underwent typical apoptotic morphologic changes such as nuclear pyknosis, chromatin condensation, and an increase of membrane permeability. Annexin V-FITC/PI double staining, cell cycle analysis, and western blot results revealed the GFP-4 induced MKN-45 cells apoptosis through the Fas/FasL-mediated death receptor pathway with cells arrested at the G0/G1 phase. These data indicate that GFP-4 is a promising candidate for treating gastric cancer and provide a theoretical basis for the future development and utilization of G. frondosa clinically.

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