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Preparation and binding specificity of antibody to 2'-5' linked triadenylic acid.

Authors
  • Shinomiya, T
  • Sawai, H
Type
Published Article
Journal
Nucleic acids symposium series
Publication Date
Jan 01, 1982
Issue
11
Pages
93–96
Identifiers
PMID: 7183978
Source
Medline
License
Unknown

Abstract

Antibody to 2'-5' linked triadenylic acid, A2'p5'A2'p5'A was prepared and purified by affinity chromatography. [8-14C]-A2'p5'A2'p5'A used in immunochemical studies was synthesized by nonenzymatic template-directed condensation. The specificity of the antibody was determined by the inhibition of the binding of the radioisotope probe to the antibody by a competitor. The cross reactivity of a number of modified oligonucleotides has been evaluated in order to gain information on the structural feature involved in the binding to the antibody. The adenine moiety and 2'-5' internucleotide linkage are crucial region for the binding. The cross reactivity of the modified oligonucleotides is indicated to have relation to the biological activity of the corresponding 5'-phosphorylated oligonucleotides.

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