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[Preparation of 32P-labelled 2-methylthioadenosine di- and triphosphates].

Authors
  • Skoblov, M Iu
  • Ias'ko, M V
  • Skoblov, Iu S
Type
Published Article
Journal
Bioorganicheskaia khimiia
Publication Date
Sep 01, 1999
Volume
25
Issue
9
Pages
702–707
Identifiers
PMID: 10624561
Source
Medline
License
Unknown

Abstract

2-Methylthioadenosine 5'-mono- and diphosphates were prepared by chemical synthesis, and enzymatically phosphorylated to 5'-[beta-32P]-diphosphate and 5'-[gamma-32P]-triphosphate, respectively. They were isolated by HPLC and had activity exceeding 1000 Ci/mmol and radiochemical purity higher than 95%.

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