Three electrospun bi-layer membranes made of nylon 6, polyacrylonitrile (PAN) and polyvinylidene fluoride (PVDF) were fabricated by electrospinning. The adhesion strength between the interfaces of nanofibres layers is measured by 180° peel tests using a 10 N load cell at 10 mm/min crosshead speed. Electrospun bi-layer membrane of PVDF gave the highest peel strength value of 11 mN/mm compared to PAN and nylon 6 (less than 5 mN/mm). The adhesion strength is attributed to the polymer solidification rate of the respective polymers. Both of bi-layer PAN/PAN and nylon 6/nylon 6 gave lower peel strength values due to rapid polymer solidification thus resulted to a poor adhesion between the layers.