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Preliminary experiments for the analysis of tetrahydrotetrols of benz[a]anthracene and benzo[a]pyrene derived from their hemoglobin adducts using a coupled-column high-performance liquid chromatographic system.

Authors
  • Hackl, C1
  • Lintelmann, J
  • Sauerbrey, R
  • Kettrup, A
  • 1 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Okologische Chemie, Oberschleissheim, Germany. , (Germany)
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Apr 12, 1996
Volume
730
Issue
1-2
Pages
153–159
Identifiers
PMID: 8680587
Source
Medline
License
Unknown

Abstract

Improved technologies for the detection of polycyclic aromatic hydrocarbon adducts are required for human biomonitoring. Therefore, a coupled-column high-performance liquid chromatographic method, with system-integrated sample processing, has been developed and its applicability for determination of tetrahydrotetrols of polycyclic aromatic hydrocarbons in acid hydrolysates of human hemoglobin has been investigated. A novel column-switching technique applying "thermotransfer' is used to separate tetrahydrotetrols of benzo[a]pyrene and benz[a]anthracene more efficiently. Derivatives of polycyclic aromatic hydrocarbons from blood hydrolysates are concentrated on a pre-column and then transferred to the analytical column by applying an electrical current to heat the solvent eluting the pre-column. This method allows for rapid and quantitative transfer of the analytes from the pre-column to the analytical column, after HPLC-integrated sample processing.

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