Affordable Access

deepdyve-link
Publisher Website

Predominant nifH transcript phylotypes related to Rhizobium rosettiformans in field-grown sugarcane plants and in Norway spruce.

Authors
  • Burbano, Claudia Sofía
  • Liu, Yuan
  • Rösner, Kim Leonie
  • Reis, Veronica Massena
  • Caballero-Mellado, Jesus
  • Reinhold-Hurek, Barbara
  • Hurek, Thomas
Type
Published Article
Journal
Environmental Microbiology Reports
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jun 01, 2011
Volume
3
Issue
3
Pages
383–389
Identifiers
DOI: 10.1111/j.1758-2229.2010.00238.x
PMID: 23761284
Source
Medline
License
Unknown

Abstract

Although some sugarcane cultivars may benefit substantially from biological nitrogen fixation (BNF), the responsible bacteria have been not identified yet. Here, we examined the active diazotrophic bacterial community in sugarcane roots from Africa and America by reverse transcription (RT)-PCR using broad-range nifH-specific primers. Denaturing gradient gel electrophoresis (DGGE) profiles obtained from sugarcane showed a low diversity at all sample locations with one phylotype amounting up to 100% of the nifH transcripts. This major phylotype has 93.9-99.6% DNA identity to the partial nifH sequence from a strain affiliated with Rhizobium rosettiformans. In addition, nifH transcripts of this phylotype were also detected in spruce roots sampled in Germany, where they made up 91% of nifH transcripts detected. In contrast, in control soil or shoot samples two distinct nifH transcript sequences distantly related to nifH from Sulfurospirillum multivorans or Bradyrhizobium elkanii, respectively, were predominant. These results suggest that R. rosettiformans is involved in root-associated nitrogen fixation with sugarcane and spruce, plants that do not form root-nodule symbioses.

Report this publication

Statistics

Seen <100 times