Practical consequences of restriction site symmetry.
- Published Article
- Publication Date
Nov 01, 1984
Because of the palindromic character of most 6-bp restriction sites, filling-in and ligation of the protruding ends create symmetric sequences which include new 6-bp restriction sites. The old site is, in most cases, lost. After cleavage at the new palindromic site and removal of the protruding ends, a new center of symmetry is created which is often part of yet another 6-bp restriction site. A compilation of potential and available sites as presented should prove useful in genetic engineering.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/6098533