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Practical consequences of restriction site symmetry.

Authors
  • Panayotatos, N
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Nov 01, 1984
Volume
31
Issue
1-3
Pages
291–294
Identifiers
PMID: 6098533
Source
Medline
License
Unknown

Abstract

Because of the palindromic character of most 6-bp restriction sites, filling-in and ligation of the protruding ends create symmetric sequences which include new 6-bp restriction sites. The old site is, in most cases, lost. After cleavage at the new palindromic site and removal of the protruding ends, a new center of symmetry is created which is often part of yet another 6-bp restriction site. A compilation of potential and available sites as presented should prove useful in genetic engineering.

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