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Practicable group testing method to evaluate weight/weight GMO content in maize grains.

Authors
  • Mano, Junichi
  • Yanaka, Yuka
  • Ikezu, Yoko
  • Onishi, Mari
  • Futo, Satoshi
  • Minegishi, Yasutaka
  • Ninomiya, Kenji
  • Yotsuyanagi, Yuichi
  • Spiegelhalter, Frank
  • Akiyama, Hiroshi
  • Teshima, Reiko
  • Hino, Akihiro
  • Naito, Shigehiro
  • Koiwa, Tomohiro
  • Takabatake, Reona
  • Furui, Satoshi
  • Kitta, Kazumi
Type
Published Article
Journal
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society
Publication Date
Jul 13, 2011
Volume
59
Issue
13
Pages
6856–6863
Identifiers
DOI: 10.1021/jf200212v
PMID: 21604714
Source
Medline
License
Unknown

Abstract

Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) content, compared to the actual weight/weight percentage of GM maize in the grain sample. As an alternative method, we designed and assessed a group testing strategy in which the GMO content is statistically evaluated based on qualitative analyses of multiple small pools, consisting of 20 maize kernels each. This approach enables the GMO content evaluation on a weight/weight basis, irrespective of the presence of stacked-event kernels. To enhance the method's user-friendliness in routine application, we devised an easy-to-use PCR-based qualitative analytical method comprising a sample preparation step in which 20 maize kernels are ground in a lysis buffer and a subsequent PCR assay in which the lysate is directly used as a DNA template. This method was validated in a multilaboratory collaborative trial.

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