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Power in Numbers: Single-Cell RNA-Seq Strategies to Dissect Complex Tissues

Authors
  • Birnbaum, Kenneth D.
Type
Published Article
Journal
Annual Review of Genetics
Publisher
Annual Reviews
Publication Date
Nov 23, 2018
Volume
52
Pages
203–221
Identifiers
DOI: 10.1146/annurev-genet-120417-031247
Source
Annual Reviews
Keywords
License
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Abstract

The growing scale and declining cost of single-cell RNA-sequencing (RNA-seq) now permit a repetition of cell sampling that increases the power to detect rare cell states, reconstruct developmental trajectories, and measure phenotype in new terms such as cellular variance. The characterization of anatomy and developmental dynamics has not had an equivalent breakthrough since groundbreaking advances in live fluorescent microscopy. The new resolution obtained by single-cell RNA-seq is a boon to genetics because the novel description of phenotype offers the opportunity to refine gene function and dissect pleiotropy. In addition, the recent pairing of high-throughput genetic perturbation with single-cell RNA-seq has made practical a scale of genetic screening not previously possible.

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