Affordable Access

deepdyve-link
Publisher Website

Potential protective effects of naringenin against vancomycin-induced nephrotoxicity via reduction on apoptotic and oxidative stress markers in rats.

Authors
  • Uckun, Zuhal1
  • Guzel, Sevda2
  • Canacankatan, Necmiye3
  • Yalaza, Cem4
  • Kibar, Deniz5
  • Coskun Yilmaz, Banu5
  • 1 Department of Pharmaceutical Toxicology, Mersin University, Mersin, Turkey. , (Turkey)
  • 2 Department of Pharmacognosy, Mersin University, Mersin, Turkey. , (Turkey)
  • 3 Department of Biochemistry, Mersin University, Mersin, Turkey. , (Turkey)
  • 4 Department of Medical Services and Techniques, Toros University Vocational School, Mersin, Turkey. , (Turkey)
  • 5 Department of Histology and Embryology, Mersin University, Mersin, Turkey. , (Turkey)
Type
Published Article
Journal
Drug and Chemical Toxicology
Publisher
Informa UK (Taylor & Francis)
Publication Date
Jan 01, 2020
Volume
43
Issue
1
Pages
104–111
Identifiers
DOI: 10.1080/01480545.2018.1512612
PMID: 30257567
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Vancomycin (VCM), a glycopeptide antibiotic, is a drug widely used in severe infections. However, VCM induce notable nephrotoxic side effects. Naringenin (NAR) is a natural of flavonoid and are known as strongly antioxidant, nefroprotective, antiapoptotic, and anti-inflammatory. The purpose of this study was to determine the potential protective effects of NAR against VCM-induced nephrotoxicity by measuring apoptotic and oxidative stress markers and evaluating histopathological alterations in rats. For this purpose, we used male Wistar albino rats that divided into seven groups: (i) Control [saline, intraperitoneally (i.p.)], (ii) carboxymethyl cellulose (0.5% CMC, orally), (iii) VCM (400 mg/kg, i.p.), (iv) NAR100 (100 mg/kg, orally), (v) VCM + NAR25 (25 mg/kg, orally), (vi) VCM + NAR50 (50 mg/kg, orally), and (vii) VCM + NAR100 (100 mg/kg, orally) groups. VCM administration was started one day after the first treatment of NAR and continued across 7-day. Caspase-3, -8, and-9 activities and malondialdehyde (MDA) and nitric oxide (NO) levels were measured by colorimetric methods in the kidney tissues, creatinine, and blood urea nitrogen (BUN) levels were analyzed based on ELISA in serum. Caspase-3 and -8 activities, NO levels, serum creatinine and BUN levels were significantly higher in VCM group in comparison with VCM + NAR (25, 50, and 100) groups (p < 0.05). Caspase-9 activity and MDA were significantly higher in VCM group compared to VCM + NAR (25 and 50) groups (p < 0.05). Histopathological alterations in VCM group were significantly diminished by administration of NAR, especially NAR 25. In conclusion, NAR 25 and 50 mg have more potent protective effects on VCM-induced nephrotoxicity compared to NAR 100 mg.

Report this publication

Statistics

Seen <100 times