Affordable Access

Access to the full text

A Potential Involvement of Anandamide in the Modulation of HO/NOS Systems: Women, Menopause, and “Medical Cannabinoids”

Authors
  • Szabó, Renáta1, 2
  • Börzsei, Denise1
  • Szabó, Zsuzsanna1
  • Hoffmann, Alexandra1
  • Zupkó, István
  • Priksz, Dániel
  • Kupai, Krisztina1
  • Varga, Csaba1
  • Pósa, Anikó1, 2
  • 1 (C.V.)
  • 2 Interdisciplinary Excellence Centre, Department of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, Hungary
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Nov 20, 2020
Volume
21
Issue
22
Identifiers
DOI: 10.3390/ijms21228801
PMID: 33233803
PMCID: PMC7699915
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Green

Abstract

Endocannabinoids and their receptors are present in the cardiovascular system; however, their actions under different pathological conditions remain controversial. The aim of our study was to examine the effects of anandamide (AEA) on heme oxygenase (HO) and nitric oxide synthase (NOS) systems in an estrogen-depleted rat model. Sham-operated (SO) and surgically induced estrogen-deficient (OVX) female Wistar rats were used. During a two-week period, a group of OVX rats received 0.1 mg/kg estrogen (E2) per os, while AEA-induced alterations were analyzed after two weeks of AEA treatment at the dose of 1.0 mg/kg. At the end of the experiment, cardiac activity and expression of HO and NOS enzymes, content of cannabinoid 1 receptor, as well as concentrations of transient potential vanilloid 1 (TRPV1) and calcitonin gene-related peptide (CGRP) were measured. Our results show that estrogen withdrawal caused a significant decrease in both NOS and HO systems, and a similar tendency was observed regarding the TRPV1/CGRP pathway. Two weeks of either AEA or E2 treatment restored the adverse changes; however, the combined administration of these two molecules did not result in a further improvement. In light of the potential relationship between AEA and HO/NOS systems, AEA-induced upregulation of HO/NOS enzymes may be a therapeutic strategy in estrogen-deficient conditions.

Report this publication

Statistics

Seen <100 times