Affordable Access

deepdyve-link
Publisher Website

The potential and challenges of nanopore sequencing.

Authors
  • D, Branton
  • Dw, Deamer
  • A, Marziali
  • H, Bayley
  • Sa, Benner
  • T, Butler
  • M, Di Ventra
  • S, Garaj
  • A, Hibbs
  • X, Huang
  • Sb, Jovanovich
  • Ps, Krstic
  • S, Lindsay
  • Xs, Ling
  • Ch, Mastrangelo
  • A, Meller
  • Js, Oliver
  • Yv, Pershin
  • Jm, Ramsey
  • R, Riehn
  • And 5 more
Type
Published Article
Journal
Nature Biotechnology
Publisher
Springer Nature
Volume
26
Issue
10
Pages
1146–1146
Identifiers
DOI: 10.1038/nbt.1495
Source
UCSC Bioengineering biomedical-ucsc
License
Unknown

Abstract

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of third generation instruments that will sequence a diploid mammalian genome for approximately $1,000 in approximately 24 h.

Report this publication

Statistics

Seen <100 times