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Potential of Auraptene in Improvement of Oocyte Maturation, Fertilization Rate, and Inflammation in Polycystic Ovary Syndrome Mouse Model.

Authors
  • Abizadeh, Marzieh1
  • Novin, Marefat Ghaffari1
  • Amidi, Fardin2
  • Ziaei, Seyed Ali3
  • Abdollahifar, Mohammad Amin1
  • Nazarian, Hamid4, 5
  • 1 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , (Iran)
  • 2 Department of Biology and Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 3 Department of Pharmaceutical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , (Iran)
  • 4 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. [email protected] , (Iran)
  • 5 Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. [email protected] , (Iran)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Sep 01, 2020
Volume
27
Issue
9
Pages
1742–1751
Identifiers
DOI: 10.1007/s43032-020-00168-9
PMID: 32124396
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Polycystic ovary with poor-quality oocytes has remained problematic in polycystic ovary syndrome (PCOS) patients. It is well documented that the inflammation and production of reactive oxygen species (ROS) in PCOS ovaries are significantly higher than normal voluntaries. In this study, we hypothesized that auraptene (AUR), as a coumarin derivative with anti-inflammatory properties, may be effective in improvement of oocyte maturation and fertilization rate in PCOS patients. For this purpose, PCOS model was induced in NMRI mice and confirmed by ovarian histopathology observations and hormonal assays. PCOS-induced mice were administrated with AUR (PCOS-AUR) and metformin (PCOS-MET), and their effects on inflammation, apoptosis rate, oocyte maturation, and in vitro fertilization capacity were determined and compared with those normal and PCOS animals treated with sesame oil (PCOS-sesame oil) and no treatment (PCOS). Treatment with AUR and MET decreased the inflammation and apoptosis rates in PCOS mice compared with PCOS animals with no treatment. PCOS-AUR and PCOS-MET oocytes also showed higher intracellular glutathione and lower ROS concentrations compared with PCOS mice, indicating improved oocyte maturation rate. PCOS-AUR and PCOS-MET groups showed higher percentages of expansion rate and MII stage oocytes, and lower rate of abnormal oocytes compared with PCOS with no treatment. The rate of fertilization in the oocytes isolated from PCOS-AUR and PCOS-MET groups was higher than PCOS-sesame oil and PCOS groups. Our findings suggest that AUR can be considered as a potential candidate for improvement of oocyte maturation and fertilization capacity in PCOS patients, comparable to MET.

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