Affordable Access

deepdyve-link
Publisher Website

Potent In Vitro Protection Against PM₂.₅-Caused ROS Generation and Vascular Permeability by Long-Term Pretreatment with Ganoderma tsugae.

Authors
  • Tseng, Chia-Yi1, 2
  • Chung, Meng-Chi2
  • Wang, Jhih-Syuan2
  • Chang, Yu-Jung2
  • Chang, Jing-Fen2
  • Lin, Chin-Hung2
  • Hseu, Ruey-Shyang3
  • Chao, Ming-Wei4, 2
  • 1 * Department of Biomedical Engineering.
  • 2 ‡ Department of Bioscience Technology, Chung Yuan Christian University Taoyuan City 32023, Taiwan. , (Taiwan)
  • 3 § Department of Biochemical Science and Technology, National Taiwan University, Da-an District, Taipei 10617, Taiwan. , (Taiwan)
  • 4 † Center of Nanotechnology.
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2016
Volume
44
Issue
2
Pages
355–376
Identifiers
DOI: 10.1142/S0192415X16500208
PMID: 27080945
Source
Medline
Keywords
License
Unknown

Abstract

Epidemiological studies show increased particulate matter (PM[Formula: see text]) particles in ambient air are correlated with increased myocardial infarctions. Given the close association of capillaries and alveoli, the dysfunction is caused when inhaled PM[Formula: see text] particles come in close proximity to capillary endothelial cells. We previously suggested that the inhalation of PM[Formula: see text] diesel exhaust particles (DEP) induces oxidative stress and upregulates the Nrf2/HO-1 pathway, inducing vascular permeability factor VEGFA secretion, which results in cell-cell adherens junction disruption and PM[Formula: see text] transmigratation into circulation. Here, we minimized the level that PM[Formula: see text] traveled in the bloodstream by pre-supplementing with a traditional Chinese medicine (TCM) Ganoderma tsugae DMSO extract (GTDE) prior to PM[Formula: see text] exposure. Our results show that PM[Formula: see text] caused alterations in enzyme activities and cellular anti-oxidant balance. We found decreased glutathione levels, a reduced cellular redox ratio, increased ROS generation and cytotoxicity in the cellular fractions. The oxidative stress caused DNA damage and apoptosis, likely causing downstream molecular events that trigger vasculature permeabilization and, eventually, cardiovascular disorders. Our results show long-term GTDE treatment increased endogenous glutathione level, while PM[Formula: see text]-reduced glutathione levels and the cellular redox ratio. GTDE was protective against the genotoxic and apoptotic effects initiated by PM[Formula: see text] oxidative stress. Vascular permeability revealed that PM[Formula: see text] only accumulated on the surface of cells after GTDE treatment; no penetration was detected. After two weeks of GTDE treatment, VEGFA secretion was significantly reduced in human umbilical vein endothelial cells (HUVEC) and endothelial cell migration was blocked. Our results suggest GTDE prevents PM[Formula: see text] transmigration into the bloodstream, and the resultant dysfunction, by inhibiting oxidative stress production and endothelial permeability.

Report this publication

Statistics

Seen <100 times